Journal: Journal of visualized experiments : JoVE

Article Title: Inducing post-traumatic epilepsy in a mouse model of repetitive diffuse traumatic brain injury

doi: 10.3791/60360

Figure Lengend Snippet: Table of materials

Article Snippet: Name of Material/Equipment Company Catalog Number Comments/Description 0.10” screw Pinnacle Technology Inc., KS, USA 8209 0.10 inch long stainless steel 0.10” screw Pinnacle Technology Inc., KS, USA 8403 0.10 inch long with pre-soldered wire lead 0.12” screw Pinnacle Technology Inc., KS, USA 8212 0.12 inch long stainless steel 1EEG headmount Invitro1 (subsidiary of Plastics One), VA, USA MS333/8-A/SPC 3 individually Teflon-insulated platinum iridium wire electrodes (twisted or untwisted, 0.005 inch diameter) extending below threaded plastic pedestal 2EEG/1EMG headmount Pinnacle Technology Inc., KS, USA 8201 2EEG/1EMG channels 3% hydrogen peroxide Pharmacy 3EEG headmount Pinnacle Technology Inc., KS, USA 8235-SM-C custom 6-Pin Connector for 3EEG channels Buprenorphine Par Pharmaceuticals, Cos. Inc., Spring Valley, NY, USA 060969 Buprenorphine Par Pharmaceuticals, Cos. Inc., Spring Valley, NY, USA 060969 C57BL/6 mice Harlan/Envigo Laboratories Inc male, 12–16 weeks old C57BL/6 mice The Jackson Laboratory male, 12–16 weeks old Carprofen Zoetis Services LLC, Parsippany, NJ, USA 026357 NOTE: this drug is added during weight drop only if stereotactic electrode implantation will be performed on the same day Chlorhexidine antiseptic Pharmacy Dental cement and solvent kit Stoelting Co., USA 51459 Drill Foredom HP4–917 Drill bit Meisinger USA, LLC, USA HM1–005-HP 0.5 mm, Round, 1/4, Steel Dry sterilizer Cellpoint Scientific, USA Germinator 500 EEG System 1 Pinnacle Technology Inc., KS, USA EEG System 2 Pinnacle Technology Inc., KS, USA Ethanol ≥70% VWR, USA 71001–652 KOPTEC USP, Biotechnology Grade (140 Proof) Eye ointment Pro Labs Ltd, USA Puralube Vet Ointment Sterile Ocular Lubricant available in general online stores and pharmacies Fluriso liquid for inhalation anesthesia MWI Veterinary Supply Co., USA 502017 Hair removal product Church & Dwight Co., Inc., USA Nair cream Isoflurane MWI Veterinary Supply Co., USA 502017 Povidone-iodine surgical solution Purdue Products, USA 004677 Betadine Rimadyl/Carprofen Zoetis Services LLC, Parsippany, NJ, USA 026357 Solder Harware store Soldering iron Weller, USA WP35 ST7 tip, 0.8mm Stainless steel disc Custom made Sterile cotton swabs Sterile gauze pads Fisher Scientific, USA 22362178 Sterile poly-lined absorbent towels pads Cardinal Health, USA 3520 Tissue adhesive 3M Animal Care Products, USA 1469SB Open in a separate window Table of materials.

Techniques: Solvent, Ointment, Sterility, Cream, Adhesive

Journal: Journal of visualized experiments : JoVE

Article Title: Inducing post-traumatic epilepsy in a mouse model of repetitive diffuse traumatic brain injury

doi: 10.3791/60360

Figure Lengend Snippet: Top panel – spontaneous seizure in a mouse 23 days after repeated weight drop TBI. Data acquired using 1EEG headmount. A – pre-ictal (pre-seizure) activity; B – ictal (seizure) activity; C – post-ictal (post-seizure) depression. Bottom panel – power spectrum density is calculated using custom script and software (see table of materials). Mean power – The average power of the power spectrum within the epoch (units: V2/Hz). Median frequency – frequency at which 50% of the total power within the epoch is reached (units: Hz). Mean frequency – frequency at which the average power within the epoch is reached (units: Hz). Spectral edge – frequency below which a user-specified percentage of the total power within the epoch is reached (units: Hz). Peak frequency – frequency at which the maximum power occurs during the epoch.

Article Snippet: Name of Material/Equipment Company Catalog Number Comments/Description 0.10” screw Pinnacle Technology Inc., KS, USA 8209 0.10 inch long stainless steel 0.10” screw Pinnacle Technology Inc., KS, USA 8403 0.10 inch long with pre-soldered wire lead 0.12” screw Pinnacle Technology Inc., KS, USA 8212 0.12 inch long stainless steel 1EEG headmount Invitro1 (subsidiary of Plastics One), VA, USA MS333/8-A/SPC 3 individually Teflon-insulated platinum iridium wire electrodes (twisted or untwisted, 0.005 inch diameter) extending below threaded plastic pedestal 2EEG/1EMG headmount Pinnacle Technology Inc., KS, USA 8201 2EEG/1EMG channels 3% hydrogen peroxide Pharmacy 3EEG headmount Pinnacle Technology Inc., KS, USA 8235-SM-C custom 6-Pin Connector for 3EEG channels Buprenorphine Par Pharmaceuticals, Cos. Inc., Spring Valley, NY, USA 060969 Buprenorphine Par Pharmaceuticals, Cos. Inc., Spring Valley, NY, USA 060969 C57BL/6 mice Harlan/Envigo Laboratories Inc male, 12–16 weeks old C57BL/6 mice The Jackson Laboratory male, 12–16 weeks old Carprofen Zoetis Services LLC, Parsippany, NJ, USA 026357 NOTE: this drug is added during weight drop only if stereotactic electrode implantation will be performed on the same day Chlorhexidine antiseptic Pharmacy Dental cement and solvent kit Stoelting Co., USA 51459 Drill Foredom HP4–917 Drill bit Meisinger USA, LLC, USA HM1–005-HP 0.5 mm, Round, 1/4, Steel Dry sterilizer Cellpoint Scientific, USA Germinator 500 EEG System 1 Pinnacle Technology Inc., KS, USA EEG System 2 Pinnacle Technology Inc., KS, USA Ethanol ≥70% VWR, USA 71001–652 KOPTEC USP, Biotechnology Grade (140 Proof) Eye ointment Pro Labs Ltd, USA Puralube Vet Ointment Sterile Ocular Lubricant available in general online stores and pharmacies Fluriso liquid for inhalation anesthesia MWI Veterinary Supply Co., USA 502017 Hair removal product Church & Dwight Co., Inc., USA Nair cream Isoflurane MWI Veterinary Supply Co., USA 502017 Povidone-iodine surgical solution Purdue Products, USA 004677 Betadine Rimadyl/Carprofen Zoetis Services LLC, Parsippany, NJ, USA 026357 Solder Harware store Soldering iron Weller, USA WP35 ST7 tip, 0.8mm Stainless steel disc Custom made Sterile cotton swabs Sterile gauze pads Fisher Scientific, USA 22362178 Sterile poly-lined absorbent towels pads Cardinal Health, USA 3520 Tissue adhesive 3M Animal Care Products, USA 1469SB Open in a separate window Table of materials.

Techniques: Activity Assay, Software